59.4 Folate:B12
59.4.1 Global tests
Type III ANOVA on main and interaction effects, and LM model summaries.
Prolactin| Chisq | Df | Pr(>Chisq) | ||
|---|---|---|---|---|
| (Intercept) | 169.8724552 | 1 | <0.0001 | *** |
| folate_B12 | 3.7847549 | 1 | 0.05 | ns |
| GDM_new | 0.5764352 | 1 | 0.4 | ns |
| Study | 2.6871647 | 1 | 0.1 | ns |
| GA_samp | 151.7582805 | 1 | <0.0001 | *** |
| folate_B12:GDM_new | 0.0111970 | 1 | 0.9 | ns |
| GDM_new:Study | 1.1101316 | 1 | 0.3 | ns |
| folate_B12:Study | 5.7254015 | 1 | 0.02 | * |
| folate_B12:GDM_new:Study | 0.0207807 | 1 | 0.9 | ns |
| Chisq | Df | Pr(>Chisq) | ||
|---|---|---|---|---|
| (Intercept) | 37.8071254 | 1 | <0.0001 | *** |
| folate_B12 | 0.0821847 | 1 | 0.8 | ns |
| GDM_new | 0.5570061 | 1 | 0.5 | ns |
| Study | 9.9200537 | 1 | 0.002 | ** |
| GA_samp | 1959.9141332 | 1 | <0.0001 | *** |
| folate_B12:GDM_new | 5.6137682 | 1 | 0.02 | * |
| GDM_new:Study | 1.5557746 | 1 | 0.2 | ns |
| folate_B12:Study | 0.0319745 | 1 | 0.9 | ns |
| folate_B12:GDM_new:Study | 5.9889500 | 1 | 0.01 | * |
| Chisq | Df | Pr(>Chisq) | ||
|---|---|---|---|---|
| (Intercept) | 325.1881724 | 1 | <0.0001 | *** |
| folate_B12 | 1.0321016 | 1 | 0.3 | ns |
| GDM_new | 0.0016102 | 1 | 1.0 | ns |
| Study | 0.1084480 | 1 | 0.7 | ns |
| GA_samp | 877.5072246 | 1 | <0.0001 | *** |
| folate_B12:GDM_new | 2.4568213 | 1 | 0.1 | ns |
| GDM_new:Study | 0.0027551 | 1 | 1.0 | ns |
| folate_B12:Study | 0.0171659 | 1 | 0.9 | ns |
| folate_B12:GDM_new:Study | 2.8020725 | 1 | 0.09 | ns |
| model term | df1 | df2 | F.ratio | Chisq | p.value | ||
|---|---|---|---|---|---|---|---|
| 1 | folate_B12 | 1 | Inf | 0.344 | 0.344 | 0.6 | ns |
| 5 | GDM_new | 1 | Inf | 28.664 | 28.664 | <0.0001 | *** |
| 7 | Study | 1 | Inf | 8.069 | 8.069 | 0.005 | ** |
| 8 | GA_samp | 1 | Inf | 0.082 | 0.082 | 0.8 | ns |
| 2 | folate_B12:GDM_new | 1 | Inf | 0.000 | 0.000 | 1.0 | ns |
| 4 | folate_B12:Study | 1 | Inf | 0.038 | 0.038 | 0.8 | ns |
| 6 | GDM_new:Study | 1 | Inf | 0.602 | 0.602 | 0.4 | ns |
| 3 | folate_B12:GDM_new:Study | 1 | Inf | 0.282 | 0.282 | 0.6 | ns |
| model term | df1 | df2 | F.ratio | Chisq | p.value | ||
|---|---|---|---|---|---|---|---|
| 1 | folate_B12 | 1 | Inf | 0.646 | 0.646 | 0.4 | ns |
| 5 | GDM_new | 1 | Inf | 29.220 | 29.220 | <0.0001 | *** |
| 7 | Study | 1 | Inf | 14.790 | 14.790 | 0.0001 | *** |
| 8 | GA_samp | 1 | Inf | 0.003 | 0.003 | 1.0 | ns |
| 2 | folate_B12:GDM_new | 1 | Inf | 0.057 | 0.057 | 0.8 | ns |
| 4 | folate_B12:Study | 1 | Inf | 0.000 | 0.000 | 1.0 | ns |
| 6 | GDM_new:Study | 1 | Inf | 0.886 | 0.886 | 0.3 | ns |
| 3 | folate_B12:GDM_new:Study | 1 | Inf | 0.162 | 0.162 | 0.7 | ns |
| model term | df1 | df2 | F.ratio | Chisq | p.value | ||
|---|---|---|---|---|---|---|---|
| 1 | folate_B12 | 1 | Inf | 0.869 | 0.869 | 0.4 | ns |
| 5 | GDM_new | 1 | Inf | 5.757 | 5.757 | 0.02 | * |
| 7 | Study | 1 | Inf | 25.701 | 25.701 | <0.0001 | *** |
| 8 | GA_samp | 1 | Inf | 0.401 | 0.401 | 0.5 | ns |
| 2 | folate_B12:GDM_new | 1 | Inf | 1.596 | 1.596 | 0.2 | ns |
| 4 | folate_B12:Study | 1 | Inf | 0.626 | 0.626 | 0.4 | ns |
| 6 | GDM_new:Study | 1 | Inf | 1.077 | 1.077 | 0.3 | ns |
| 3 | folate_B12:GDM_new:Study | 1 | Inf | 0.045 | 0.045 | 0.8 | ns |
59.4.2 Model summary
Ratio of Geometric means (95% CI)
Prolactin| Var | Effect | P |
|---|---|---|
| (Intercept) | 2.69 (2.29, 3.09) | <0.0001 |
| folate_B12 | -0.00 (-0.00, -0.00) | 0.04 |
| GDM_newGDM | -0.11 (-0.34, 0.13) | 0.37 |
| StudySTOP | -0.13 (-0.29, 0.02) | 0.10 |
| GA_samp | 0.15 (0.13, 0.17) | <0.0001 |
| folate_B12:GDM_newGDM | -0.00 (-0.00, 0.00) | 0.95 |
| GDM_newGDM:StudySTOP | 0.17 (-0.06, 0.40) | 0.15 |
| folate_B12:StudySTOP | 0.00 (0.00, 0.00) | 0.01 |
| Var | Effect | P |
|---|---|---|
| (Intercept) | 0.66 (0.45, 0.87) | <0.0001 |
| folate_B12 | -0.00 (-0.00, 0.00) | 0.77 |
| GDM_newGDM | 0.06 (-0.10, 0.22) | 0.46 |
| StudySTOP | 0.14 (0.05, 0.22) | 0.002 |
| GA_samp | 0.29 (0.28, 0.30) | <0.0001 |
| folate_B12:GDM_newGDM | -0.00 (-0.00, -0.00) | 0.02 |
| GDM_newGDM:StudySTOP | -0.11 (-0.29, 0.06) | 0.21 |
| folate_B12:StudySTOP | -0.00 (-0.00, 0.00) | 0.86 |
| folate_B12:GDM_newGDM:StudySTOP | 0.00 (0.00, 0.00) | 0.01 |
| Var | Effect | P |
|---|---|---|
| (Intercept) | -2.55 (-2.82, -2.27) | <0.0001 |
| folate_B12 | -0.00 (-0.00, 0.00) | 0.10 |
| GDM_newGDM | -0.14 (-0.31, 0.03) | 0.11 |
| StudySTOP | -0.04 (-0.15, 0.07) | 0.43 |
| GA_samp | 0.26 (0.24, 0.28) | <0.0001 |
| folate_B12:GDM_newGDM | -0.00 (-0.00, 0.00) | 0.89 |
| GDM_newGDM:StudySTOP | 0.17 (0.00, 0.34) | 0.05 |
| folate_B12:StudySTOP | 0.00 (-0.00, 0.00) | 0.37 |
| Var | Effect | P |
|---|---|---|
| (Intercept) | 2.60 (2.07, 3.13) | <0.0001 |
| folate_B12 | -0.00 (-0.00, 0.00) | 0.66 |
| GDM_newGDM | 0.84 (0.50, 1.19) | <0.0001 |
| StudySTOP | -0.31 (-0.52, -0.10) | 0.004 |
| GA_samp | 0.00 (-0.03, 0.04) | 0.77 |
| folate_B12:GDM_newGDM | -0.00 (-0.00, 0.00) | 0.72 |
| GDM_newGDM:StudySTOP | -0.27 (-0.61, 0.07) | 0.12 |
| folate_B12:StudySTOP | 0.00 (-0.00, 0.00) | 0.88 |
| Var | Effect | P |
|---|---|---|
| (Intercept) | 1.24 (0.68, 1.81) | <0.0001 |
| folate_B12 | -0.00 (-0.00, 0.00) | 0.56 |
| GDM_newGDM | 0.95 (0.57, 1.33) | <0.0001 |
| StudySTOP | -0.47 (-0.69, -0.25) | <0.0001 |
| GA_samp | 0.00 (-0.03, 0.04) | 0.96 |
| folate_B12:GDM_newGDM | -0.00 (-0.00, 0.00) | 0.58 |
| GDM_newGDM:StudySTOP | -0.33 (-0.71, 0.04) | 0.08 |
| folate_B12:StudySTOP | 0.00 (-0.00, 0.00) | 0.75 |
| Var | Effect | P |
|---|---|---|
| (Intercept) | 1.73 (1.61, 1.84) | <0.0001 |
| folate_B12 | -0.00 (-0.00, 0.00) | 0.64 |
| GDM_newGDM | 0.15 (0.01, 0.28) | 0.03 |
| StudySTOP | -0.16 (-0.20, -0.11) | <0.0001 |
| GA_samp | -0.00 (-0.01, 0.00) | 0.53 |
| folate_B12:GDM_newGDM | -0.00 (-0.00, 0.00) | 0.15 |
| GDM_newGDM:StudySTOP | -0.08 (-0.20, 0.04) | 0.17 |
| folate_B12:StudySTOP | 0.00 (-0.00, 0.00) | 0.28 |
59.4.3 Estimated means
Estimated marginal means (or adjusted means) adjusted for GA at sampling.
Results are averaged over the levels of Folate:B12 ratio and may be misleading when interaction term is involved.
| Study | GDM_new | response | SE | df | lower.CL | upper.CL |
|---|---|---|---|---|---|---|
| SCOPE | Uncomplicated | 94.29984 | 7.625504 | 102.8302 | 80.32660 | 110.7038 |
| STOP | Uncomplicated | 93.42616 | 6.988326 | 75.6675 | 80.49400 | 108.4360 |
| SCOPE | GDM | 84.46162 | 10.602255 | 465.7427 | 65.99816 | 108.0904 |
| STOP | GDM | 98.93897 | 8.704386 | 140.0553 | 83.14349 | 117.7353 |
| Study | GDM_new | response | SE | df | lower.CL | upper.CL |
|---|---|---|---|---|---|---|
| SCOPE | Uncomplicated | 86.17832 | 2.538979 | 188.2151 | 81.31257 | 91.33523 |
| STOP | Uncomplicated | 98.38177 | 2.495652 | 104.5259 | 93.55549 | 103.45702 |
| SCOPE | GDM | 80.17900 | 4.471729 | 630.3602 | 71.86149 | 89.45920 |
| STOP | GDM | 95.49611 | 3.390919 | 309.2910 | 89.05168 | 102.40691 |
| Study | GDM_new | response | SE | df | lower.CL | upper.CL |
|---|---|---|---|---|---|---|
| SCOPE | Uncomplicated | 2.239282 | 0.0748675 | 442.0255 | 2.096872 | 2.391365 |
| STOP | Uncomplicated | 2.209041 | 0.0576251 | 202.4688 | 2.098291 | 2.325636 |
| SCOPE | GDM | 1.942566 | 0.1508117 | 1034.7015 | 1.668073 | 2.262228 |
| STOP | GDM | 2.269315 | 0.0938631 | 485.5939 | 2.092182 | 2.461444 |
| Study | GDM_new | response | SE | df | asymp.LCL | asymp.UCL |
|---|---|---|---|---|---|---|
| SCOPE | Uncomplicated | 14.07586 | 0.8800801 | Inf | 12.452432 | 15.91092 |
| STOP | Uncomplicated | 10.44075 | 0.4305347 | Inf | 9.630115 | 11.31962 |
| SCOPE | GDM | 32.03042 | 4.9978966 | Inf | 23.590902 | 43.48912 |
| STOP | GDM | 18.08465 | 1.3890691 | Inf | 15.557147 | 21.02279 |
| Study | GDM_new | response | SE | df | asymp.LCL | asymp.UCL |
|---|---|---|---|---|---|---|
| SCOPE | Uncomplicated | 3.413043 | 0.2271328 | Inf | 2.995681 | 3.888552 |
| STOP | Uncomplicated | 2.175776 | 0.0942232 | Inf | 1.998722 | 2.368514 |
| SCOPE | GDM | 8.531524 | 1.4624141 | Inf | 6.097048 | 11.938056 |
| STOP | GDM | 3.899323 | 0.3220337 | Inf | 3.316583 | 4.584452 |
| Study | GDM_new | response | SE | df | asymp.LCL | asymp.UCL |
|---|---|---|---|---|---|---|
| SCOPE | Uncomplicated | 5.428502 | 0.0689608 | Inf | 5.295011 | 5.565360 |
| STOP | Uncomplicated | 4.713930 | 0.0424742 | Inf | 4.631413 | 4.797917 |
| SCOPE | GDM | 6.085856 | 0.3238477 | Inf | 5.483105 | 6.754867 |
| STOP | GDM | 4.878311 | 0.0637282 | Inf | 4.754991 | 5.004829 |
59.4.4 Comparisons
59.4.4.1 Within Groups
Effect for every 100 unit increase in Folate:B12 ratio.| Hormone | Contrast | Study | GDM_new | Ratio of Geometric means (95% CI) | P-value |
|---|---|---|---|---|---|
| Prolactin | folate_B12245.96 / folate_B12145.96 | SCOPE | Uncomplicated | 0.961 (0.924, 0.999) | 0.04 |
| Prolactin | folate_B12245.96 / folate_B12145.96 | STOP | Uncomplicated | 1.023 (0.991, 1.055) | 0.2 |
| Prolactin | folate_B12245.96 / folate_B12145.96 | SCOPE | GDM | 0.959 (0.906, 1.016) | 0.2 |
| Prolactin | folate_B12245.96 / folate_B12145.96 | STOP | GDM | 1.021 (0.980, 1.064) | 0.3 |
| HPL | folate_B12245.96 / folate_B12145.96 | SCOPE | Uncomplicated | 0.997 (0.976, 1.018) | 0.8 |
| HPL | folate_B12245.96 / folate_B12145.96 | STOP | Uncomplicated | 0.994 (0.977, 1.012) | 0.5 |
| HPL | folate_B12245.96 / folate_B12145.96 | SCOPE | GDM | 0.932 (0.885, 0.981) | 0.008 |
| HPL | folate_B12245.96 / folate_B12145.96 | STOP | GDM | 1.006 (0.982, 1.030) | 0.6 |
| GH2 | folate_B12245.96 / folate_B12145.96 | SCOPE | Uncomplicated | 0.977 (0.950, 1.005) | 0.1 |
| GH2 | folate_B12245.96 / folate_B12145.96 | STOP | Uncomplicated | 0.993 (0.970, 1.016) | 0.5 |
| GH2 | folate_B12245.96 / folate_B12145.96 | SCOPE | GDM | 0.974 (0.934, 1.016) | 0.2 |
| GH2 | folate_B12245.96 / folate_B12145.96 | STOP | GDM | 0.990 (0.960, 1.020) | 0.5 |
| Insulin | folate_B12245.96 / folate_B12145.96 | SCOPE | Uncomplicated | 0.990 (0.944, 1.037) | 0.7 |
| Insulin | folate_B12245.96 / folate_B12145.96 | STOP | Uncomplicated | 0.994 (0.960, 1.029) | 0.7 |
| Insulin | folate_B12245.96 / folate_B12145.96 | SCOPE | GDM | 0.979 (0.918, 1.045) | 0.5 |
| Insulin | folate_B12245.96 / folate_B12145.96 | STOP | GDM | 0.983 (0.935, 1.035) | 0.5 |
| HOMA.IR | folate_B12245.96 / folate_B12145.96 | SCOPE | Uncomplicated | 0.985 (0.938, 1.035) | 0.6 |
| HOMA.IR | folate_B12245.96 / folate_B12145.96 | STOP | Uncomplicated | 0.995 (0.958, 1.033) | 0.8 |
| HOMA.IR | folate_B12245.96 / folate_B12145.96 | SCOPE | GDM | 0.968 (0.901, 1.040) | 0.4 |
| HOMA.IR | folate_B12245.96 / folate_B12145.96 | STOP | GDM | 0.977 (0.925, 1.033) | 0.4 |
| GLUC3_mmol.L | folate_B12245.96 / folate_B12145.96 | SCOPE | Uncomplicated | 0.998 (0.988, 1.007) | 0.6 |
| GLUC3_mmol.L | folate_B12245.96 / folate_B12145.96 | STOP | Uncomplicated | 1.007 (0.995, 1.018) | 0.2 |
| GLUC3_mmol.L | folate_B12245.96 / folate_B12145.96 | SCOPE | GDM | 0.982 (0.956, 1.009) | 0.2 |
| GLUC3_mmol.L | folate_B12245.96 / folate_B12145.96 | STOP | GDM | 0.991 (0.976, 1.006) | 0.2 |
59.4.4.2 Between Groups
Below are comparisons of the differences in the change in Folate:B12 ratio between groups. I.e. whether the change in Folate:B12 ratio are different between groups.
| contrast | ratio | SE | df | lower.CL | upper.CL | p.value | |
|---|---|---|---|---|---|---|---|
| SCOPE/SCOPE (Overall) | 1.1331947 | 0.0552608 | 1362.154 | 1.0298130 | 1.246955 | 0.010 | * |
| STOP/SCOPE (Uncomplicated) | 1.0645162 | 0.0259558 | 1362.154 | 1.0147970 | 1.116671 | 0.010 | * |
| STOP/SCOPE (GDM) | 1.0645162 | 0.0259558 | 1362.154 | 1.0147970 | 1.116671 | 0.010 | * |
| GDM/Uncomplicated (SCOPE) | 0.9985844 | 0.0250522 | 1343.765 | 0.9506285 | 1.048960 | 0.955 | ns |
| GDM/Uncomplicated (STOP) | 0.9985844 | 0.0250522 | 1343.765 | 0.9506285 | 1.048960 | 0.955 | ns |
| contrast | ratio | SE | df | lower.CL | upper.CL | p.value | |
|---|---|---|---|---|---|---|---|
| SCOPE/SCOPE (Overall) | 1.0762540 | 0.0345865 | 649.9090 | 1.0104376 | 1.1463574 | 0.023 | * |
| STOP/SCOPE (Uncomplicated) | 0.9974815 | 0.0140667 | 1289.1463 | 0.9702636 | 1.0254629 | 0.858 | ns |
| STOP/SCOPE (GDM) | 1.0789714 | 0.0311288 | 549.2789 | 1.0195256 | 1.1418832 | 0.009 | ** |
| GDM/Uncomplicated (SCOPE) | 0.9349310 | 0.0265494 | 504.3811 | 0.8841983 | 0.9885745 | 0.018 | * |
| GDM/Uncomplicated (STOP) | 1.0113108 | 0.0153519 | 1398.9582 | 0.9816395 | 1.0418789 | 0.459 | ns |
| contrast | ratio | SE | df | lower.CL | upper.CL | p.value | |
|---|---|---|---|---|---|---|---|
| SCOPE/SCOPE (Overall) | 1.0319994 | 0.0364042 | 1360.679 | 0.9630000 | 1.105943 | 0.372 | ns |
| STOP/SCOPE (Uncomplicated) | 1.0158737 | 0.0179177 | 1360.679 | 0.9813256 | 1.051638 | 0.372 | ns |
| STOP/SCOPE (GDM) | 1.0158737 | 0.0179177 | 1360.679 | 0.9813256 | 1.051638 | 0.372 | ns |
| GDM/Uncomplicated (SCOPE) | 0.9973472 | 0.0184678 | 1391.902 | 0.9617694 | 1.034241 | 0.886 | ns |
| GDM/Uncomplicated (STOP) | 0.9973472 | 0.0184678 | 1391.902 | 0.9617694 | 1.034241 | 0.886 | ns |
| contrast | ratio | SE | df | asymp.LCL | asymp.UCL | p.value | |
|---|---|---|---|---|---|---|---|
| SCOPE/SCOPE (Overall) | 1.0087364 | 0.0565249 | Inf | 0.9038166 | 1.125836 | 0.877 | ns |
| STOP/SCOPE (Uncomplicated) | 1.0043587 | 0.0281398 | Inf | 0.9506927 | 1.061054 | 0.877 | ns |
| STOP/SCOPE (GDM) | 1.0043587 | 0.0281398 | Inf | 0.9506927 | 1.061054 | 0.877 | ns |
| GDM/Uncomplicated (SCOPE) | 0.9894412 | 0.0289828 | Inf | 0.9342358 | 1.047909 | 0.717 | ns |
| GDM/Uncomplicated (STOP) | 0.9894412 | 0.0289828 | Inf | 0.9342358 | 1.047909 | 0.717 | ns |
| contrast | ratio | SE | df | asymp.LCL | asymp.UCL | p.value | |
|---|---|---|---|---|---|---|---|
| SCOPE/SCOPE (Overall) | 1.0197048 | 0.0615394 | Inf | 0.9059501 | 1.147743 | 0.746 | ns |
| STOP/SCOPE (Uncomplicated) | 1.0098043 | 0.0304709 | Inf | 0.9518141 | 1.071328 | 0.746 | ns |
| STOP/SCOPE (GDM) | 1.0098043 | 0.0304709 | Inf | 0.9518141 | 1.071328 | 0.746 | ns |
| GDM/Uncomplicated (SCOPE) | 0.9823047 | 0.0316369 | Inf | 0.9222141 | 1.046311 | 0.579 | ns |
| GDM/Uncomplicated (STOP) | 0.9823047 | 0.0316369 | Inf | 0.9222141 | 1.046311 | 0.579 | ns |
| contrast | ratio | SE | df | asymp.LCL | asymp.UCL | p.value | |
|---|---|---|---|---|---|---|---|
| SCOPE/SCOPE (Overall) | 1.0177933 | 0.0165485 | Inf | 0.9858703 | 1.050750 | 0.278 | ns |
| STOP/SCOPE (Uncomplicated) | 1.0088574 | 0.0082016 | Inf | 0.9929100 | 1.025061 | 0.278 | ns |
| STOP/SCOPE (GDM) | 1.0088574 | 0.0082016 | Inf | 0.9929100 | 1.025061 | 0.278 | ns |
| GDM/Uncomplicated (SCOPE) | 0.9844365 | 0.0108104 | Inf | 0.9634748 | 1.005854 | 0.153 | ns |
| GDM/Uncomplicated (STOP) | 0.9844365 | 0.0108104 | Inf | 0.9634748 | 1.005854 | 0.153 | ns |