59.4 Folate:B12

59.4.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

Prolactin
Chisq Df Pr(>Chisq)
(Intercept) 169.8724552 1 <0.0001 ***
folate_B12 3.7847549 1 0.05 ns
GDM_new 0.5764352 1 0.4 ns
Study 2.6871647 1 0.1 ns
GA_samp 151.7582805 1 <0.0001 ***
folate_B12:GDM_new 0.0111970 1 0.9 ns
GDM_new:Study 1.1101316 1 0.3 ns
folate_B12:Study 5.7254015 1 0.02 *
folate_B12:GDM_new:Study 0.0207807 1 0.9 ns
HPL
Chisq Df Pr(>Chisq)
(Intercept) 37.8071254 1 <0.0001 ***
folate_B12 0.0821847 1 0.8 ns
GDM_new 0.5570061 1 0.5 ns
Study 9.9200537 1 0.002 **
GA_samp 1959.9141332 1 <0.0001 ***
folate_B12:GDM_new 5.6137682 1 0.02 *
GDM_new:Study 1.5557746 1 0.2 ns
folate_B12:Study 0.0319745 1 0.9 ns
folate_B12:GDM_new:Study 5.9889500 1 0.01 *
GH2
Chisq Df Pr(>Chisq)
(Intercept) 325.1881724 1 <0.0001 ***
folate_B12 1.0321016 1 0.3 ns
GDM_new 0.0016102 1 1.0 ns
Study 0.1084480 1 0.7 ns
GA_samp 877.5072246 1 <0.0001 ***
folate_B12:GDM_new 2.4568213 1 0.1 ns
GDM_new:Study 0.0027551 1 1.0 ns
folate_B12:Study 0.0171659 1 0.9 ns
folate_B12:GDM_new:Study 2.8020725 1 0.09 ns
Insulin
model term df1 df2 F.ratio Chisq p.value
1 folate_B12 1 Inf 0.344 0.344 0.6 ns
5 GDM_new 1 Inf 28.664 28.664 <0.0001 ***
7 Study 1 Inf 8.069 8.069 0.005 **
8 GA_samp 1 Inf 0.082 0.082 0.8 ns
2 folate_B12:GDM_new 1 Inf 0.000 0.000 1.0 ns
4 folate_B12:Study 1 Inf 0.038 0.038 0.8 ns
6 GDM_new:Study 1 Inf 0.602 0.602 0.4 ns
3 folate_B12:GDM_new:Study 1 Inf 0.282 0.282 0.6 ns
HOMA.IR
model term df1 df2 F.ratio Chisq p.value
1 folate_B12 1 Inf 0.646 0.646 0.4 ns
5 GDM_new 1 Inf 29.220 29.220 <0.0001 ***
7 Study 1 Inf 14.790 14.790 0.0001 ***
8 GA_samp 1 Inf 0.003 0.003 1.0 ns
2 folate_B12:GDM_new 1 Inf 0.057 0.057 0.8 ns
4 folate_B12:Study 1 Inf 0.000 0.000 1.0 ns
6 GDM_new:Study 1 Inf 0.886 0.886 0.3 ns
3 folate_B12:GDM_new:Study 1 Inf 0.162 0.162 0.7 ns
GLUC3_mmol.L
model term df1 df2 F.ratio Chisq p.value
1 folate_B12 1 Inf 0.869 0.869 0.4 ns
5 GDM_new 1 Inf 5.757 5.757 0.02 *
7 Study 1 Inf 25.701 25.701 <0.0001 ***
8 GA_samp 1 Inf 0.401 0.401 0.5 ns
2 folate_B12:GDM_new 1 Inf 1.596 1.596 0.2 ns
4 folate_B12:Study 1 Inf 0.626 0.626 0.4 ns
6 GDM_new:Study 1 Inf 1.077 1.077 0.3 ns
3 folate_B12:GDM_new:Study 1 Inf 0.045 0.045 0.8 ns

59.4.2 Model summary

Ratio of Geometric means (95% CI)

Prolactin
Var Effect P
(Intercept) 2.69 (2.29, 3.09) <0.0001
folate_B12 -0.00 (-0.00, -0.00) 0.04
GDM_newGDM -0.11 (-0.34, 0.13) 0.37
StudySTOP -0.13 (-0.29, 0.02) 0.10
GA_samp 0.15 (0.13, 0.17) <0.0001
folate_B12:GDM_newGDM -0.00 (-0.00, 0.00) 0.95
GDM_newGDM:StudySTOP 0.17 (-0.06, 0.40) 0.15
folate_B12:StudySTOP 0.00 (0.00, 0.00) 0.01
HPL
Var Effect P
(Intercept) 0.66 (0.45, 0.87) <0.0001
folate_B12 -0.00 (-0.00, 0.00) 0.77
GDM_newGDM 0.06 (-0.10, 0.22) 0.46
StudySTOP 0.14 (0.05, 0.22) 0.002
GA_samp 0.29 (0.28, 0.30) <0.0001
folate_B12:GDM_newGDM -0.00 (-0.00, -0.00) 0.02
GDM_newGDM:StudySTOP -0.11 (-0.29, 0.06) 0.21
folate_B12:StudySTOP -0.00 (-0.00, 0.00) 0.86
folate_B12:GDM_newGDM:StudySTOP 0.00 (0.00, 0.00) 0.01
GH2
Var Effect P
(Intercept) -2.55 (-2.82, -2.27) <0.0001
folate_B12 -0.00 (-0.00, 0.00) 0.10
GDM_newGDM -0.14 (-0.31, 0.03) 0.11
StudySTOP -0.04 (-0.15, 0.07) 0.43
GA_samp 0.26 (0.24, 0.28) <0.0001
folate_B12:GDM_newGDM -0.00 (-0.00, 0.00) 0.89
GDM_newGDM:StudySTOP 0.17 (0.00, 0.34) 0.05
folate_B12:StudySTOP 0.00 (-0.00, 0.00) 0.37
Insulin
Var Effect P
(Intercept) 2.60 (2.07, 3.13) <0.0001
folate_B12 -0.00 (-0.00, 0.00) 0.66
GDM_newGDM 0.84 (0.50, 1.19) <0.0001
StudySTOP -0.31 (-0.52, -0.10) 0.004
GA_samp 0.00 (-0.03, 0.04) 0.77
folate_B12:GDM_newGDM -0.00 (-0.00, 0.00) 0.72
GDM_newGDM:StudySTOP -0.27 (-0.61, 0.07) 0.12
folate_B12:StudySTOP 0.00 (-0.00, 0.00) 0.88
HOMA.IR
Var Effect P
(Intercept) 1.24 (0.68, 1.81) <0.0001
folate_B12 -0.00 (-0.00, 0.00) 0.56
GDM_newGDM 0.95 (0.57, 1.33) <0.0001
StudySTOP -0.47 (-0.69, -0.25) <0.0001
GA_samp 0.00 (-0.03, 0.04) 0.96
folate_B12:GDM_newGDM -0.00 (-0.00, 0.00) 0.58
GDM_newGDM:StudySTOP -0.33 (-0.71, 0.04) 0.08
folate_B12:StudySTOP 0.00 (-0.00, 0.00) 0.75
GLUC3_mmol.L
Var Effect P
(Intercept) 1.73 (1.61, 1.84) <0.0001
folate_B12 -0.00 (-0.00, 0.00) 0.64
GDM_newGDM 0.15 (0.01, 0.28) 0.03
StudySTOP -0.16 (-0.20, -0.11) <0.0001
GA_samp -0.00 (-0.01, 0.00) 0.53
folate_B12:GDM_newGDM -0.00 (-0.00, 0.00) 0.15
GDM_newGDM:StudySTOP -0.08 (-0.20, 0.04) 0.17
folate_B12:StudySTOP 0.00 (-0.00, 0.00) 0.28

59.4.3 Estimated means

Estimated marginal means (or adjusted means) adjusted for GA at sampling.

Results are averaged over the levels of Folate:B12 ratio and may be misleading when interaction term is involved.

Prolactin
Study GDM_new response SE df lower.CL upper.CL
SCOPE Uncomplicated 94.29984 7.625504 102.8302 80.32660 110.7038
STOP Uncomplicated 93.42616 6.988326 75.6675 80.49400 108.4360
SCOPE GDM 84.46162 10.602255 465.7427 65.99816 108.0904
STOP GDM 98.93897 8.704386 140.0553 83.14349 117.7353
HPL
Study GDM_new response SE df lower.CL upper.CL
SCOPE Uncomplicated 86.17832 2.538979 188.2151 81.31257 91.33523
STOP Uncomplicated 98.38177 2.495652 104.5259 93.55549 103.45702
SCOPE GDM 80.17900 4.471729 630.3602 71.86149 89.45920
STOP GDM 95.49611 3.390919 309.2910 89.05168 102.40691
GH2
Study GDM_new response SE df lower.CL upper.CL
SCOPE Uncomplicated 2.239282 0.0748675 442.0255 2.096872 2.391365
STOP Uncomplicated 2.209041 0.0576251 202.4688 2.098291 2.325636
SCOPE GDM 1.942566 0.1508117 1034.7015 1.668073 2.262228
STOP GDM 2.269315 0.0938631 485.5939 2.092182 2.461444
Insulin
Study GDM_new response SE df asymp.LCL asymp.UCL
SCOPE Uncomplicated 14.07586 0.8800801 Inf 12.452432 15.91092
STOP Uncomplicated 10.44075 0.4305347 Inf 9.630115 11.31962
SCOPE GDM 32.03042 4.9978966 Inf 23.590902 43.48912
STOP GDM 18.08465 1.3890691 Inf 15.557147 21.02279
HOMA.IR
Study GDM_new response SE df asymp.LCL asymp.UCL
SCOPE Uncomplicated 3.413043 0.2271328 Inf 2.995681 3.888552
STOP Uncomplicated 2.175776 0.0942232 Inf 1.998722 2.368514
SCOPE GDM 8.531524 1.4624141 Inf 6.097048 11.938056
STOP GDM 3.899323 0.3220337 Inf 3.316583 4.584452
GLUC3_mmol.L
Study GDM_new response SE df asymp.LCL asymp.UCL
SCOPE Uncomplicated 5.428502 0.0689608 Inf 5.295011 5.565360
STOP Uncomplicated 4.713930 0.0424742 Inf 4.631413 4.797917
SCOPE GDM 6.085856 0.3238477 Inf 5.483105 6.754867
STOP GDM 4.878311 0.0637282 Inf 4.754991 5.004829

59.4.4 Comparisons

59.4.4.1 Within Groups

Effect for every 100 unit increase in Folate:B12 ratio.
Hormone Contrast Study GDM_new Ratio of Geometric means (95% CI) P-value
Prolactin folate_B12245.96 / folate_B12145.96 SCOPE Uncomplicated 0.961 (0.924, 0.999) 0.04
Prolactin folate_B12245.96 / folate_B12145.96 STOP Uncomplicated 1.023 (0.991, 1.055) 0.2
Prolactin folate_B12245.96 / folate_B12145.96 SCOPE GDM 0.959 (0.906, 1.016) 0.2
Prolactin folate_B12245.96 / folate_B12145.96 STOP GDM 1.021 (0.980, 1.064) 0.3
HPL folate_B12245.96 / folate_B12145.96 SCOPE Uncomplicated 0.997 (0.976, 1.018) 0.8
HPL folate_B12245.96 / folate_B12145.96 STOP Uncomplicated 0.994 (0.977, 1.012) 0.5
HPL folate_B12245.96 / folate_B12145.96 SCOPE GDM 0.932 (0.885, 0.981) 0.008
HPL folate_B12245.96 / folate_B12145.96 STOP GDM 1.006 (0.982, 1.030) 0.6
GH2 folate_B12245.96 / folate_B12145.96 SCOPE Uncomplicated 0.977 (0.950, 1.005) 0.1
GH2 folate_B12245.96 / folate_B12145.96 STOP Uncomplicated 0.993 (0.970, 1.016) 0.5
GH2 folate_B12245.96 / folate_B12145.96 SCOPE GDM 0.974 (0.934, 1.016) 0.2
GH2 folate_B12245.96 / folate_B12145.96 STOP GDM 0.990 (0.960, 1.020) 0.5
Insulin folate_B12245.96 / folate_B12145.96 SCOPE Uncomplicated 0.990 (0.944, 1.037) 0.7
Insulin folate_B12245.96 / folate_B12145.96 STOP Uncomplicated 0.994 (0.960, 1.029) 0.7
Insulin folate_B12245.96 / folate_B12145.96 SCOPE GDM 0.979 (0.918, 1.045) 0.5
Insulin folate_B12245.96 / folate_B12145.96 STOP GDM 0.983 (0.935, 1.035) 0.5
HOMA.IR folate_B12245.96 / folate_B12145.96 SCOPE Uncomplicated 0.985 (0.938, 1.035) 0.6
HOMA.IR folate_B12245.96 / folate_B12145.96 STOP Uncomplicated 0.995 (0.958, 1.033) 0.8
HOMA.IR folate_B12245.96 / folate_B12145.96 SCOPE GDM 0.968 (0.901, 1.040) 0.4
HOMA.IR folate_B12245.96 / folate_B12145.96 STOP GDM 0.977 (0.925, 1.033) 0.4
GLUC3_mmol.L folate_B12245.96 / folate_B12145.96 SCOPE Uncomplicated 0.998 (0.988, 1.007) 0.6
GLUC3_mmol.L folate_B12245.96 / folate_B12145.96 STOP Uncomplicated 1.007 (0.995, 1.018) 0.2
GLUC3_mmol.L folate_B12245.96 / folate_B12145.96 SCOPE GDM 0.982 (0.956, 1.009) 0.2
GLUC3_mmol.L folate_B12245.96 / folate_B12145.96 STOP GDM 0.991 (0.976, 1.006) 0.2

59.4.4.2 Between Groups

Below are comparisons of the differences in the change in Folate:B12 ratio between groups. I.e. whether the change in Folate:B12 ratio are different between groups.

Prolactin
contrast ratio SE df lower.CL upper.CL p.value
SCOPE/SCOPE (Overall) 1.1331947 0.0552608 1362.154 1.0298130 1.246955 0.010 *
STOP/SCOPE (Uncomplicated) 1.0645162 0.0259558 1362.154 1.0147970 1.116671 0.010 *
STOP/SCOPE (GDM) 1.0645162 0.0259558 1362.154 1.0147970 1.116671 0.010 *
GDM/Uncomplicated (SCOPE) 0.9985844 0.0250522 1343.765 0.9506285 1.048960 0.955 ns
GDM/Uncomplicated (STOP) 0.9985844 0.0250522 1343.765 0.9506285 1.048960 0.955 ns
HPL
contrast ratio SE df lower.CL upper.CL p.value
SCOPE/SCOPE (Overall) 1.0762540 0.0345865 649.9090 1.0104376 1.1463574 0.023 *
STOP/SCOPE (Uncomplicated) 0.9974815 0.0140667 1289.1463 0.9702636 1.0254629 0.858 ns
STOP/SCOPE (GDM) 1.0789714 0.0311288 549.2789 1.0195256 1.1418832 0.009 **
GDM/Uncomplicated (SCOPE) 0.9349310 0.0265494 504.3811 0.8841983 0.9885745 0.018 *
GDM/Uncomplicated (STOP) 1.0113108 0.0153519 1398.9582 0.9816395 1.0418789 0.459 ns
GH2
contrast ratio SE df lower.CL upper.CL p.value
SCOPE/SCOPE (Overall) 1.0319994 0.0364042 1360.679 0.9630000 1.105943 0.372 ns
STOP/SCOPE (Uncomplicated) 1.0158737 0.0179177 1360.679 0.9813256 1.051638 0.372 ns
STOP/SCOPE (GDM) 1.0158737 0.0179177 1360.679 0.9813256 1.051638 0.372 ns
GDM/Uncomplicated (SCOPE) 0.9973472 0.0184678 1391.902 0.9617694 1.034241 0.886 ns
GDM/Uncomplicated (STOP) 0.9973472 0.0184678 1391.902 0.9617694 1.034241 0.886 ns
Insulin
contrast ratio SE df asymp.LCL asymp.UCL p.value
SCOPE/SCOPE (Overall) 1.0087364 0.0565249 Inf 0.9038166 1.125836 0.877 ns
STOP/SCOPE (Uncomplicated) 1.0043587 0.0281398 Inf 0.9506927 1.061054 0.877 ns
STOP/SCOPE (GDM) 1.0043587 0.0281398 Inf 0.9506927 1.061054 0.877 ns
GDM/Uncomplicated (SCOPE) 0.9894412 0.0289828 Inf 0.9342358 1.047909 0.717 ns
GDM/Uncomplicated (STOP) 0.9894412 0.0289828 Inf 0.9342358 1.047909 0.717 ns
HOMA.IR
contrast ratio SE df asymp.LCL asymp.UCL p.value
SCOPE/SCOPE (Overall) 1.0197048 0.0615394 Inf 0.9059501 1.147743 0.746 ns
STOP/SCOPE (Uncomplicated) 1.0098043 0.0304709 Inf 0.9518141 1.071328 0.746 ns
STOP/SCOPE (GDM) 1.0098043 0.0304709 Inf 0.9518141 1.071328 0.746 ns
GDM/Uncomplicated (SCOPE) 0.9823047 0.0316369 Inf 0.9222141 1.046311 0.579 ns
GDM/Uncomplicated (STOP) 0.9823047 0.0316369 Inf 0.9222141 1.046311 0.579 ns
GLUC3_mmol.L
contrast ratio SE df asymp.LCL asymp.UCL p.value
SCOPE/SCOPE (Overall) 1.0177933 0.0165485 Inf 0.9858703 1.050750 0.278 ns
STOP/SCOPE (Uncomplicated) 1.0088574 0.0082016 Inf 0.9929100 1.025061 0.278 ns
STOP/SCOPE (GDM) 1.0088574 0.0082016 Inf 0.9929100 1.025061 0.278 ns
GDM/Uncomplicated (SCOPE) 0.9844365 0.0108104 Inf 0.9634748 1.005854 0.153 ns
GDM/Uncomplicated (STOP) 0.9844365 0.0108104 Inf 0.9634748 1.005854 0.153 ns

59.4.5 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

59.4.6 Plots